In chromatography, purity begins before the first injection.
One of the most overlooked yet critical steps is the filtration of buffer solutions used in the mobile phase.

Why is it important?

Removes particulate matter: Prevents column blockage, protects pump seals, and extends instrument life.

Enhances baseline stability: Eliminates micro-particles that can cause noise or ghost peaks.

Improves reproducibility: Ensures consistent flow rates and separation efficiency.


Ideal Filter Papers for Buffer Filtration

When filtering aqueous buffer solutions, it’s crucial to use the right membrane to avoid leaching or chemical incompatibility. Common choices:

Nylon (0.45 µm or 0.2 µm) – Excellent for most aqueous buffers; low extractables.

PTFE (0.45 µm or 0.2 µm) – Best for organic solvent compatibility; hydrophobic but can be pre-wetted for aqueous use.

Cellulose Nitrate / Cellulose Acetate (0.45 µm) – Low protein binding, suitable for biological samples.

Glass Fiber Pre-filters – Ideal for high-particulate buffers before membrane filtration.


Best Practice Tip: Always degas after filtration to prevent bubble formation inside the filter and ensure a smooth mobile phase flow.




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