All Questions
votes
answers
views
IC choromaphy
Hello everyone, I am a new lab technician, and I'm analyzing anions with an IC equipment such as Cloride, nitrate, sulfate. My peak data has pea...
votes
answers
views
PEAK GOT FRONTING
Please help me with this I am using ammonium acetate pH 5.5: ACN (40:60) 1ml/min I tried changing all the parameters but still getting fronting...
votes
answers
views
votes
answers
views
Request a service manual
I would be happy to receive the service manual for the NMR DD2 Platform if you have it available. Please send it to me at the following email address:...
votes
answers
views
Baseline drift
What could be causing this drift in baseline, I've tried changing mobile phase, degassing, temp is stable, proper purging with mobile phase...
votes
answers
views
Lysine aescinate injection
Good morning to all. Now I'm trying to develop quantification method for lysine aescinate injection by HPLC. Lysine peak in not eluting. If someo...
votes
answers
views
Totally Porous vs. Superficially Porous HPLC columns
The Problem: The Pressure vs. Efficiency Trade-off Totally Porous Particles (The Old Standard): These are like "sponges" or "Nerf ball...
votes
answers
views
Why Assuming RRF = 1.0 Risks Toxic Impurity Errors
In HPLC analysis, the biggest lie a chromatogram can tell you is that "bigger peak = more amount." We all know that different molecules i...
votes
answers
views
Is Your HPLC Method "Green" Enough?
Sustainability in the lab is no longer just a buzzword—in 2025, it’s a necessity. With rising solvent costs and stricter waste regulations, Green ...
votes
answers
views
Suggest Mobile Phase for Sertraline HCl Impurity Method for, with good separation between Racemic Mixture and Sertraline HCl
Suggest Mobile Phase for Sertraline HCl Impurity Method for, with good separation between "USP Sertaline HCl Racemic Mixture and Sertraline HCl&...