PEAK GOT FRONTING
Please help me with this
I am using ammonium acetate pH 5.5: ACN
(40:60)
1ml/min
I tried changing all the parameters but still getting fronting
1 Answers
Your Answer
Log in to answer this question.
2
Peak fronting is due to sample overloading and solvent mismatch (high ACN content).
It can be minimized by reducing injection volume, diluting sample, and preparing sample in mobile phase.
It can be minimized by reducing injection volume, diluting sample, and preparing sample in mobile phase.
