All Questions | HPLC Calculator

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HPLC calibration questions and answers

1. Pump Calibration Q1. What is the acceptance criterion for flow rate accuracy? A1. The flow rate should be within ±2.0% of the set value. Q2. How...

youhana Hosny asked 1 year ago

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235
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In reversed-phase HPLC, if you observe peak broadening and tailing for a basic analyte, which mobile phase adjustment is most likely to improve peak shape?

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HPLC hplc

john asked 1 year ago

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289
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You are tasked with developing an HPLC method for analyzing the molecule in the photo. Which of the following is the most appropriate combination of column and detector?

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HPLC hplc method_development

youhana Hosny asked 1 year ago

2
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2
answers

172
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Signal to noise ratio

Hi there We have shimdazu lab solution softwear . Where signal to ratio calculation is difficult. Someone who can guide how to calculate ?...

HPLC

kumar purushottam asked 1 year ago

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177
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custom calculations with Shimadzu Lab solutions

Can we do custom calculations with Shimadzu Lab solutions software? If yes what are the steps to be followed?...

HPLC

vijay kumar Yeedibilli asked 1 year ago

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138
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Condroitín sulfate & Glucosamine sulfate

Someone has to work with condroitín and Glucosamine sulfato, HPLC PDA 195 nm ?...

HPLC

Gabriela Chalen asked 1 year ago

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421
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Which parameter is a measure of column efficiency in HPLC?

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HPLC column_efficiency hplc

youhana Hosny asked 1 year ago

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447
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What type of HPLC techniques do you mostly use?

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HPLC hplc

youhana Hosny asked 1 year ago

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2
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256
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Peak to valley ratio

What is the possible causes that affect peak to valley ratio? Standard preparation? System? Column? But I'm using a new column...

HPLC

Kashvenaa Santhiran asked 1 year ago

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240
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Zoledronic acid peak

Does anyone know the perfect mobile phase for zoledronic acid peak detection in hplc?...

HPLC

Atiya Hashim asked 1 year ago