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Method finetuning

How do i turn off the lamp automatically after my last peak of interest is eluted off, to save lamptime

HPLC dad lamp method
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Avatar Diksha Wani 11 m ago

if you have gap of 2-4hrs in between two injections, it may save lamp on time if u turn the lamp off, but not the life of lamp as number of ignitions also matters for long life of lamp.

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Avatar youhana Hosny 11 m ago

If you want to do it between injections it's not recommended but you can do it after sequence completion in the wash method you can easily turn off the lamp.

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Toham Basha 11 m ago
turn it off when your run completes
Avatar Sarah 11 m ago

What kind of system do you have?

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Frequently Asked Questions: HPLC Analysis & Chromatography

High-Performance Liquid Chromatography (HPLC) is an analytical technique used to separate, identify, and quantify each component in a mixture. It relies on a pump to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out of the column.

Column efficiency is typically measured by the number of Theoretical Plates ($N$). The most common formula is $N = 16 \times (t_r / W)^2$, where $t_r$ is the retention time and $W$ is the peak width at the base. A higher number of theoretical plates indicates a sharper peak and better analytical separation. You can calculate this instantly using our Theoretical Plates Calculator.

The ICH (International Council for Harmonisation) Q2(R1) guidelines mandate specific validation parameters for HPLC methods. These include assessing Accuracy, Precision (Repeatability and Intermediate Precision), Specificity, Detection Limit (LOD), Quantitation Limit (LOQ), Linearity, and Range. Our calculators are designed specifically to help analysts easily compute these linearity, LOD/LOQ, and %RSD parameters in compliance with ICH standards.
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