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Orthogonality in RP-LC

Have you ever applied this approach when testing selectivity during analytical method validation? I've red about this sometimes, but I usually don't use this approach for selectivity testing while developing, I tend to use more they clasic PDA-assisted spectral evaluation of impurities profile.

HPLC development orthogonality validation
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Avatar youhana Hosny 2 m ago

First time to hear about it

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