All Questions
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Why Spike Impurities Below LOQ in Method Validation (Related Substances – Accuracy Studies)?
During HPLC method validation for related substances, spiking impurities at levels below the LOQ may seem unnecessary—since values below LOQ are “...
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HPLC baseline noise
When using Acetonitrile and water composition in the ratio 50:50 premixed and sonicated for 10mins and ran one injection of blank,it's look like ...
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Assay Simeticone
Hello, good morning. Can anyone help me a method for quantifying Simeticone using reverse-phase HPLC? Thanks you i'm avance...
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Importance of Filtration of Buffer Solution in Mobile Phase Preparation
In chromatography, purity begins before the first injection. One of the most overlooked yet critical steps is the filtration of buffer solutions used...
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HPLC Base Line Issue
In my HPLC Agelent 1260 infinity model, base line going to upward direction and my buffer is Ammonium Bicarbonate with pH - 8 and mixed with MeOH. Run...
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How to Read and Interpret FTIR Spectra — A Complete Guide??
FTIR (Fourier Transform Infrared Spectroscopy) is a fundamental technique in analytical chemistry, materials science, and nanotechnology, providing a ...
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HPLC Troubleshooting: Common Questions and Expert Answers
Q: Why is the HPLC baseline noisy? A: Common reasons include air bubbles in the system, an aging detector lamp, a contaminated flow cell, or impuritie...
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Gas Chromatography (GC) is a powerful analytical technique, How It Works?
Gas Chromatography (GC) is a powerful analytical technique widely used to separate and analyze volatile organic compounds across a variety of sample t...
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Mastering Column Selection for Optimal Results.
“Column selection isn’t just one step in method development—it’s the cornerstone of achieving accurate, reliable separations.” Here are e...