Questions tagged [methoddevelopment]
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Why You Should Use Volatile Buffers in LC-M?
Choosing the right buffers and solvents is critical in LC-MS because the entire mobile phase directly enters the mass spectrometer. Compatibility with...
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Chirality in HPLC Method Development – Why It Matters?
When developing an HPLC method, we often focus on column chemistry, mobile phase composition, and detection. But what happens when the compound itself...
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Normal-Phase vs Reversed-Phase HPLC
One of the most important choices in chromatography method development is deciding between Normal-Phase (NP) and Reversed-Phase (RP) HPLC. The followi...
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Mastering Column Selection for Optimal Results.
“Column selection isn’t just one step in method development—it’s the cornerstone of achieving accurate, reliable separations.” Here are e...