Questions tagged [analyticalchemistry]
votes
answers
views
Totally Porous vs. Superficially Porous HPLC columns
The Problem: The Pressure vs. Efficiency Trade-off Totally Porous Particles (The Old Standard): These are like "sponges" or "Nerf ball...
votes
answers
views
Why Assuming RRF = 1.0 Risks Toxic Impurity Errors
In HPLC analysis, the biggest lie a chromatogram can tell you is that "bigger peak = more amount." We all know that different molecules i...
votes
answers
views
Is Your HPLC Method "Green" Enough?
Sustainability in the lab is no longer just a buzzword—in 2025, it’s a necessity. With rising solvent costs and stricter waste regulations, Green ...
votes
answers
views
votes
answers
views
Why You Should Use Volatile Buffers in LC-M?
Choosing the right buffers and solvents is critical in LC-MS because the entire mobile phase directly enters the mass spectrometer. Compatibility with...
votes
answers
views
Normal-Phase vs Reversed-Phase HPLC
One of the most important choices in chromatography method development is deciding between Normal-Phase (NP) and Reversed-Phase (RP) HPLC. The followi...
votes
answers
views
Carryover – The Hidden Threat to Analytical Accuracy
In analytical science, precision is non-negotiable. Yet, one silent contaminant can distort results and jeopardize data integrity — carryover. Wh...
votes
answers
views
Importance of Filtration of Buffer Solution in Mobile Phase Preparation
In chromatography, purity begins before the first injection. One of the most overlooked yet critical steps is the filtration of buffer solutions used...
votes
answers
views
How to Read and Interpret FTIR Spectra — A Complete Guide??
FTIR (Fourier Transform Infrared Spectroscopy) is a fundamental technique in analytical chemistry, materials science, and nanotechnology, providing a ...
votes
answers
views
Gas Chromatography (GC) is a powerful analytical technique, How It Works?
Gas Chromatography (GC) is a powerful analytical technique widely used to separate and analyze volatile organic compounds across a variety of sample t...