You are developing an HPLC method for a basic compound. After injecting the sample, you observe poor peak shape (fronting/tailing) and variable retention time. Which of the following is the most likely cause? (check comments for hints and learn) Poll
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Think about the pKa of basic drugs – how are they charged at low pH?
Consider the stationary phase – what happens when silanol groups are not masked?
Peak fronting/tailing + retention issues = possible secondary interactions or ionization mismatch.
Which condition increases interaction with residual silanols?
Consider the stationary phase – what happens when silanol groups are not masked?
Peak fronting/tailing + retention issues = possible secondary interactions or ionization mismatch.
Which condition increases interaction with residual silanols?
