HPLC baseline noise
When using Acetonitrile and water composition in the ratio 50:50 premixed and sonicated for 10mins and ran one injection of blank,it's look like noisy baseline.so using nylon filter and used still te noise occurs.
I used this mobile phase in RS test with the run time of 70mins .
Give me a suggestion to reduce the noise by using this mobile phase.
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1. Check column if there is any bubble, keep the open from outlet and rotate it and let the mobile phase pass for 10-15 mins.
2. Give back pressure from the outlet of Detector.
3. Equilibrate system for atleast 1 hr.
2. Give back pressure from the outlet of Detector.
3. Equilibrate system for atleast 1 hr.
