All Questions
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HPLC Calibration – Key Parameters and Acceptance Criteria
High-Performance Liquid Chromatography (HPLC) is one of the most critical analytical techniques in the pharmaceutical industry. To ensure accuracy, re...
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LC-MS vs GC-MS in Pharmaceutical Analysis
1. Principle LC-MS (Liquid Chromatography–Mass Spectrometry): Separates analytes in the liquid phase using HPLC and detects them with a mass spect...
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Normal-Phase vs Reversed-Phase HPLC
One of the most important choices in chromatography method development is deciding between Normal-Phase (NP) and Reversed-Phase (RP) HPLC. The followi...
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Carryover – The Hidden Threat to Analytical Accuracy
In analytical science, precision is non-negotiable. Yet, one silent contaminant can distort results and jeopardize data integrity — carryover. Wh...
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Why Spike Impurities Below LOQ in Method Validation (Related Substances – Accuracy Studies)?
During HPLC method validation for related substances, spiking impurities at levels below the LOQ may seem unnecessary—since values below LOQ are “...
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HPLC baseline noise
When using Acetonitrile and water composition in the ratio 50:50 premixed and sonicated for 10mins and ran one injection of blank,it's look like ...
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Assay Simeticone
Hello, good morning. Can anyone help me a method for quantifying Simeticone using reverse-phase HPLC? Thanks you i'm avance...
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Importance of Filtration of Buffer Solution in Mobile Phase Preparation
In chromatography, purity begins before the first injection. One of the most overlooked yet critical steps is the filtration of buffer solutions used...
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HPLC Base Line Issue
In my HPLC Agelent 1260 infinity model, base line going to upward direction and my buffer is Ammonium Bicarbonate with pH - 8 and mixed with MeOH. Run...
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How to Read and Interpret FTIR Spectra — A Complete Guide??
FTIR (Fourier Transform Infrared Spectroscopy) is a fundamental technique in analytical chemistry, materials science, and nanotechnology, providing a ...