All Questions
LOD &LOQ
What will be the concentration of any API for LOD ,& LOQ estimation , if signal to noise ratio concentration is 0.001% ?...
Ghost Peaks Appearing Randomly
While running a long sequence, I started seeing ghost peaks appear randomly in blanks and sometimes in unrelated samples. At first, I thought it wa...
Ion Suppression in LC-MS/MS
I was analyzing plasma samples using LC-MS/MS for a drug quantification study. The standard curve looked great in pure solvent, but when I injected ex...
Unexpected Peaks After Running a Solvent Blank
I was running a sequence of product samples and decided to inject a methanol blank in between. To my surprise, I saw unexpected peaks—some of which ...
Retention Time Shifts Mid-Run
In a long GC run (about 30 samples), I noticed that retention times started shifting gradually. Early samples were fine, but later ones drifted....
Mobile phase issue ?
Have you ever experienced a situation where the apparent pH of your buffered mobile phase changes significantly over time, even when stored in a seale...
Peak shape issue ?
Have you observed peak tailing or fronting that is specific to certain analytes and doesn't respond to typical mobile phase adjustments ?...
