Analysis syrup contain vit D3 and Calcium
Now I try to analysis syrup contain Vit.D3 and calcium by hplc but i have difficulty separating to get clear result as assay
Can you give any method or tips
2 Answers
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0
Use mobile phase of (MeOH 96%:ACN 4%), and use solvent methane 100% measure at 265 nm with C18, 15 cm column with a flow rate of 1.5 ml/min will give you a good peak for Vitamin D3
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use CH3OH:H20 (90:10) as MP for Vit.D3 & go for titration method for Ca
