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No peaks found on the chromatogram.

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Avatar youhana Hosny 2 m ago

Possible Causes:

Detector not connected or turned on

Leak in the system

Injection volume too low

Column not properly equilibrated

Solutions:

Verify the detector settings and signal connection

Check for leaks at fittings and seals

Increase injection volume or concentration

Allow the column to equilibrate with the mobile phase for at least 10-20 column volumes

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