No Peaks Appearing in Chromatogram
No peaks found on the chromatogram.
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Accepted Answer
Possible Causes:
Detector not connected or turned on
Leak in the system
Injection volume too low
Column not properly equilibrated
Solutions:
Verify the detector settings and signal connection
Check for leaks at fittings and seals
Increase injection volume or concentration
Allow the column to equilibrate with the mobile phase for at least 10-20 column volumes
Detector not connected or turned on
Leak in the system
Injection volume too low
Column not properly equilibrated
Solutions:
Verify the detector settings and signal connection
Check for leaks at fittings and seals
Increase injection volume or concentration
Allow the column to equilibrate with the mobile phase for at least 10-20 column volumes