All Questions
votes
answers
views
Why Assuming RRF = 1.0 Risks Toxic Impurity Errors
In HPLC analysis, the biggest lie a chromatogram can tell you is that "bigger peak = more amount." We all know that different molecules i...
votes
answers
views
Is Your HPLC Method "Green" Enough?
Sustainability in the lab is no longer just a buzzword—in 2025, it’s a necessity. With rising solvent costs and stricter waste regulations, Green ...
votes
answers
views
Suggest Mobile Phase for Sertraline HCl Impurity Method for, with good separation between Racemic Mixture and Sertraline HCl
Suggest Mobile Phase for Sertraline HCl Impurity Method for, with good separation between "USP Sertaline HCl Racemic Mixture and Sertraline HCl&...
votes
answers
views
Suggest Naproxen Sodium UV Spectroscopy Assay Method
Required naproxen sodium Assay Alternate method in UV Spectro. Diluent=? UV spectro parameters=?...
votes
answers
views
HPLC PRESSURE DROP
During batch file run after five std. Run pressure drop occurs due to which shift in RT. How can we file an incident report. What reason and explanati...
votes
answers
views
The Critical Role of pKa in HPLC Method Development
In analytical method development, particularly for analyzing active pharmaceutical ingredients (APIs) and their impurities, the pKa value of a compoun...
votes
answers
views
Uv
Please inform me of UV calibration with total perameter and it's procese...
votes
answers
views
Suggest Mobile phase for Impurity of Oteseconazole:SHR8008X:SHR8008JA retention time with good separation
Suggest Mobile: Composition isocrated / Gradient for Oteseconazole:SHR8008X:SHR8008JA retention time with good separation....
votes
answers
views
