Lysine aescinate injection

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Good morning to all. Now I'm trying to develop quantification method for lysine aescinate injection by HPLC. Lysine peak in not eluting. If someone knows about these method parameters, please help me.

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1
Primesep 100 column with UV 210 nm it is working only lysine standard.
0
it can’t be detected on uv detector
are using uv detector ?