All Questions
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Simultaneous estimation of rp-hplc of two drugs
Can you tell how can we calculate the peak height of any peak of hplc...
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Method finetuning
How do i turn off the lamp automatically after my last peak of interest is eluted off, to save lamptime...
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HPLC Calibration: The Heartbeat of Reliable Pharmaceutical Analysis
High-Performance Liquid Chromatography (HPLC) is more than just a tool — it's the backbone of accurate pharmaceutical testing. But without prop...
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Which part of HPLC method development do you find most challenging?
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LOD &LOQ
What will be the concentration of any API for LOD ,& LOQ estimation , if signal to noise ratio concentration is 0.001% ?...
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Ghost Peaks Appearing Randomly
While running a long sequence, I started seeing ghost peaks appear randomly in blanks and sometimes in unrelated samples. At first, I thought it wa...
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Ion Suppression in LC-MS/MS
I was analyzing plasma samples using LC-MS/MS for a drug quantification study. The standard curve looked great in pure solvent, but when I injected ex...
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Unexpected Peaks After Running a Solvent Blank
I was running a sequence of product samples and decided to inject a methanol blank in between. To my surprise, I saw unexpected peaks—some of which ...