Meaning of k prime and void volume
For calculation of k prime and
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K prime simply means by how many times of unretained peak (solvent peak) also called void time can the column retain the main peak (T-T0/T0)
while the void volume is the volume of mobile phase required to elute the unretained peak (solvent peak).
For the flow rate of 1 ml/min the retention time of the unretained peak (solvent peak) also called void time T0 is equal to the void volume
while the void volume is the volume of mobile phase required to elute the unretained peak (solvent peak).
For the flow rate of 1 ml/min the retention time of the unretained peak (solvent peak) also called void time T0 is equal to the void volume
