Questions in HPLC
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Understanding chromatographic resolution (Rs)
Understanding chromatographic resolution (Rs) is crucial for accurate analysis! ? Watch how two overlapping peaks separate to achieve baseline resolut...
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IC choromaphy
Hello everyone, I am a new lab technician, and I'm analyzing anions with an IC equipment such as Cloride, nitrate, sulfate. My peak data has pea...
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PEAK GOT FRONTING
Please help me with this I am using ammonium acetate pH 5.5: ACN (40:60) 1ml/min I tried changing all the parameters but still getting fronting...
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Baseline drift
What could be causing this drift in baseline, I've tried changing mobile phase, degassing, temp is stable, proper purging with mobile phase...
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Lysine aescinate injection
Good morning to all. Now I'm trying to develop quantification method for lysine aescinate injection by HPLC. Lysine peak in not eluting. If someo...
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Totally Porous vs. Superficially Porous HPLC columns
The Problem: The Pressure vs. Efficiency Trade-off Totally Porous Particles (The Old Standard): These are like "sponges" or "Nerf ball...
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Why Assuming RRF = 1.0 Risks Toxic Impurity Errors
In HPLC analysis, the biggest lie a chromatogram can tell you is that "bigger peak = more amount." We all know that different molecules i...
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Is Your HPLC Method "Green" Enough?
Sustainability in the lab is no longer just a buzzword—in 2025, it’s a necessity. With rising solvent costs and stricter waste regulations, Green ...