Questions in HPLC
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Lysine aescinate injection
Good morning to all. Now I'm trying to develop quantification method for lysine aescinate injection by HPLC. Lysine peak in not eluting. If someo...
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Totally Porous vs. Superficially Porous HPLC columns
The Problem: The Pressure vs. Efficiency Trade-off Totally Porous Particles (The Old Standard): These are like "sponges" or "Nerf ball...
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Why Assuming RRF = 1.0 Risks Toxic Impurity Errors
In HPLC analysis, the biggest lie a chromatogram can tell you is that "bigger peak = more amount." We all know that different molecules i...
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Is Your HPLC Method "Green" Enough?
Sustainability in the lab is no longer just a buzzword—in 2025, it’s a necessity. With rising solvent costs and stricter waste regulations, Green ...
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Suggest Mobile Phase for Sertraline HCl Impurity Method for, with good separation between Racemic Mixture and Sertraline HCl
Suggest Mobile Phase for Sertraline HCl Impurity Method for, with good separation between "USP Sertaline HCl Racemic Mixture and Sertraline HCl&...
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HPLC PRESSURE DROP
During batch file run after five std. Run pressure drop occurs due to which shift in RT. How can we file an incident report. What reason and explanati...
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The Critical Role of pKa in HPLC Method Development
In analytical method development, particularly for analyzing active pharmaceutical ingredients (APIs) and their impurities, the pKa value of a compoun...
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Suggest Mobile phase for Impurity of Oteseconazole:SHR8008X:SHR8008JA retention time with good separation
Suggest Mobile: Composition isocrated / Gradient for Oteseconazole:SHR8008X:SHR8008JA retention time with good separation....
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