Babak Astani 1 m ago

Hi
Diluent:dissolve 13.6 g KH2PO4 in 2000 ml water and adjust pH to 6.8 with NaOH.
Standard Solution:
25 mg of Naproxen in 50 ml, first add 5 ml Methanol and add Diluent to 50ml. after that 5 ml of this solution transfer to 100 ml and fill with water.
Test solution:
weight Equivalent 250 mg naproxen in 100 ml, add 5 ml Methanol to Dissolve Naproxen after that add diluent to volume 100ml.then transfer 1ml of solution to volumetric flask and add water to volume 100.
Blank is water.
wavelength: 263nm

youhana Hosny 2 m ago

if you can use the same diluent as your starting mobile phase composition

Muhammad Syahrul 2 m ago

In general, several parameters in UV-Vis and HPLC calibration include:Wavelength accuracy, Wavelength linearity, Noise & drift