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Why is my peak tailing in the chromatogram?
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2 m ago
My peak shows tailing how can I solve it?
HPLC
youhana Hosny
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3 Answers
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Use end-capped or well-deactivated columns.
Optimize pH, especially for ionizable analytes.
Add modifiers (e.g., TEA) to reduce interactions.
Inject smaller sample volumes.
Replace or regenerate the column if damaged.
0
good answers thanks :)
-1
how can I calulate tailing factor