Why Proper Column Storage Is Essential ?

After completing your HPLC runs—especially when using salt-containing mobile phases—taking the right steps in column care is key to preserving performance and extending the column’s lifespan.

This visual outlines the best practices for column storage:
✔️ Using salt-containing mobile phases? Always flush with a salt-free solvent before storage.
✔️ Using non-salt mobile phases? You can go straight to the wash step.
✅ In both cases, finish with a wash using 100% organic solvent (e.g., acetonitrile), then seal the column with end plugs.

? The second part highlights how column quality dramatically affects chromatographic performance:
• Well-end-capped, inert columns deliver sharp, symmetrical peaks.
• Poorly end-capped columns result in distorted peaks, even after flushing.

? Column chemistry, cleaning protocols, and storage conditions all play a vital role in achieving high-resolution, reproducible results and optimal sensitivity.

? What’s your post-analysis storage routine? Do you follow a flushing protocol every time?