chromatogram ciprofloxacin

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49 views 7 d ago

can yoi help me to be sure that this is the real retention time of this compound? if not? what is the real retention time.
Colun: 4,6 x 150mm
mobile phase : A) TFA 0,025 % + water, and B) Acetonitril + TFA
Time: 12 min
inj: 10 ųl
debit: 0,5 ml/min
267 nm
temperature: 30°c

HPLC
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the compound i found is ciprofloxacin.
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the peaks are not well separated