Types of HPLC Columns –What to Use and When
Column choice isn't just about C18! ?
From amino to phenyl, each stationary phase brings something unique to your separation.
✅ Here's a visual guide to help you choose the right column based on:
• Analyte polarity
• Functional groups
• Interaction types
• Application fields (e.g., sugars, peptides, phenolics, pharmaceuticals)
? Choosing the wrong column can cost you resolution, time, and sample integrity. Don’t waste injections — get it right the first time!
? What’ss your favorite go-to column, and what do you use it for?
Post by Ahmed Newehy