Pressure

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I have agilent 1260 infinity II instrument and i found that pressure is reaching 400 without installation of column , what is the problem ? and how to solve it ?

HPLC
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Ibrahim Author
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I believe the problem is with tubing you have to check the tubes one by one to know which one is blocked and needs to be replaced
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Check the each tube. If it was not then change the flit in pressure compartment & flush the system with magic solution with 0.2 ml/min flow for 8 hours.