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Method Transfer (HPLC ↔ UPLC)
Instantly scale flow rate, injection volume, and run time for new column dimensions
Isocratic Method
Gradient Method
Original Method (HPLC)
Length L
1
(mm)
*
ID
1
(mm)
*
Particle Size dp
1
(µm)
*
Flow Rate
1
(mL/min)
*
Injection Vol
1
(µL)
*
Run Time
1
(min)
*
Gradient Table
1
*
Time (min)
% A
% B
✖
+ Add Step
Target Method (UPLC)
Length L
2
(mm)
*
ID
2
(mm)
*
Particle Size dp
2
(µm)
*
Scaled Method Parameters
Flow Rate
—
mL/min
Inj. Vol
—
µL
Run Time
—
min
Calculated Target Gradient
Time (min)
% A
% B
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Transfer Component Scaling Rules
Flow Rate =
F
1
* (ID
2
/ ID
1
)² * (dp
1
/ dp
2
)
Inj. Volume =
V
1
* (L
2
* ID
2
²) / (L
1
* ID
1
²)
Time (Isocratic & Gradient Steps) =
t
1
* (L
2
/ L
1
) * (ID
2
/ ID
1
)² * (F
1
/ F
2
)